Journal: Scientific Reports

Article Title: Identified endoplasmic reticulum stress-related molecular cluster and immune characterization in endometriosis

doi: 10.1038/s41598-025-22400-9

Figure Lengend Snippet: qRT-PCR and IHC validation the hub gene expression level. ( a ) Hub ERS genes mRNA expression of normal, eutopic and ectopic endometrium. ( b ) Immunostaining of EPAS1, F8, VCAM1, VWF protein expression. ( c ) Semi-quantitative analysis of IHC results. qRT-PCR, real-time quantitative reverse transcription; IHC, immunohistochemistry. * p < 0.05, ** p < 0.01, ∗ ∗ ∗ p < 0.001, **** p < 0.0001.

Article Snippet: Briefly, the sections were stained with the primary antibody against F8 (1:500, affinity, USA), VCAM1(1:200, Proteintech, Wuhan, China), VWF (1:400, Proteintech, Wuhan, China), and EPAS1(1:50, Proteintech, Wuhan, China) and then scanned with a digital scanner.

Techniques: Quantitative RT-PCR, Biomarker Discovery, Gene Expression, Expressing, Immunostaining, Reverse Transcription, Immunohistochemistry

Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: Long non-coding RNA and mRNA profile analysis of metformin to reverse the pulmonary hypertension vascular remodeling induced by monocrotaline.

doi: 10.1016/j.biopha.2019.108933

Figure Lengend Snippet: Fig. 4. Signal pathway identification and transcription factor activity verification. A: Representative western blotting strips of PAs from MCT-induced PAH rats with or without metformin treatment and densitometric analysis showing Hif1α, PKG relative to β-actin; B: Representative western blotting strips of PAs from MCT- induced PAH rats with or without metformin treatment and densitometric analysis showing p-AKT relative to AKT; C: Representative western blotting strips of PAs from MCT-induced PAH rats with or without metformin treatment and densitometric analysis showing p53 relative to β-actin; D: Representative western blotting strips of PAs from MCT-induced PAH rats with or without metformin treatment and densitometric analysis showing Sp1, Est1 relative to β-actin. MCT means monocrotaline; Met means metformin. **p < 0.01. n = 3.

Article Snippet: The dilution ratio of all primary antibodies used was as follows: (PKG, catalog number: 21646-1-AP, 1:1000 dilution, proteintech), (Sp1, catalog number: 21962-1-AP, 1:2000 dilution, proteintech), (Est1, catalog number: 12522-1-AP, 1:1000 dilution, proteintech), (Hif1α, Cat. #: AF1009, 1:2000 dilution, Affinity Biosciences), (p53, catalog number: 10442-1-AP, 1:5000 dilution, proteintech), (Cyclin D1, catalog number: 26755-1-AP, 1:1000 dilution, proteintech), (β-actin, catalog number: 60008-1-1 g, 1:10000 dilution, proteintech), (AKT, catalog number: 10176-2-AP, 1:2000 dilution, proteintech), (p-AKT, # 9271, 1:1000 dilution, Cell Signaling Technology).

Techniques: Activity Assay, Western Blot

Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: Long non-coding RNA and mRNA profile analysis of metformin to reverse the pulmonary hypertension vascular remodeling induced by monocrotaline.

doi: 10.1016/j.biopha.2019.108933

Figure Lengend Snippet: Fig. 7. Mechanism analysis of NONRATT015587.2 regulation of pulmonary vascular remodeling in vitro assay and differential gene clustergram in MCT-induced PAH rats and controls, or metformin treatment. A-G: Realtime-PCR analysis of p53, Est1, Rela, Cebpa/b, Sp1, Hif1α expression in pcDNA, pcDNA- NONRATT015587.2, and pcDNA- NONRATT015587.2+metformin group. H: Representative western blotting strips of PASMCs from pcDNA-NONRATT015587.2 with or without metformin treatment and densitometric analysis showing Hif1α, p53 relative to β-actin; I: The differential expression of mRNA in the microarray analysis. Hierarchical clustering analysis is shown for 32 mRNA (fold change > 2, p < 0.05) in the control, monocrotaline and monocrotaline + metformin group using gene microarray analysis. MCT means monocrotaline; Met means metformin. **p < 0.01. n = 3.

Article Snippet: The dilution ratio of all primary antibodies used was as follows: (PKG, catalog number: 21646-1-AP, 1:1000 dilution, proteintech), (Sp1, catalog number: 21962-1-AP, 1:2000 dilution, proteintech), (Est1, catalog number: 12522-1-AP, 1:1000 dilution, proteintech), (Hif1α, Cat. #: AF1009, 1:2000 dilution, Affinity Biosciences), (p53, catalog number: 10442-1-AP, 1:5000 dilution, proteintech), (Cyclin D1, catalog number: 26755-1-AP, 1:1000 dilution, proteintech), (β-actin, catalog number: 60008-1-1 g, 1:10000 dilution, proteintech), (AKT, catalog number: 10176-2-AP, 1:2000 dilution, proteintech), (p-AKT, # 9271, 1:1000 dilution, Cell Signaling Technology).

Techniques: In Vitro, Expressing, Western Blot, Quantitative Proteomics, Microarray, Control